Immunofluorescence analysis of Rat-spinal-cord tissue. 1,α-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labeled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.