Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,Desmin Monoclonal Antibody (1B12) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.