Western blot analysis of 1) Hela, 2) 293T, with CA IX Mouse mAb diluted at 1:5,000.
1、Input: Hela Cell Lysate
2、IP product: IP dilute 1:200
Western blot analysis: primary antibody : E12-101 1:2,000
Secondary antibody: Goat anti-Mouse IgG, Light chain specific(S003), 1:5,000