1ug VSV-G fusion protein+ Primary antibody dilution at 1、1:5,000 （lane 1）and 1:10,000 (lane 2).

IP antibody use：5ug VSV-G Mouse IgG1 per ml Lysate，WB 1:5000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with VSV-G-tag fusion protein 3、IP(untransfted 293+anti-VSV-G mAb+Protein G agarose) 4、IP (transfected 293+ normal Mouse IgG+Protein G agarose) 5、IP (transfected 293+anti-VSV-G mAb+ Protein G agarose) 6、IP (transfected 293+Protein G) 7、Recombinant protein (E.coli) IF analysis of 293T cells transfected with a VSV-G-tagged protein, using VSV-G-Tag (8D6) Mouse mAb at a 1:2000 dilution (blue DAPI ,red anti-VSV-G).