2ug His fusion protein+ Primary antibody dilution at 1:5,000 (lane 1) and 1:10,000 (lane 2).

IP antibody use：5ug His Mouse IgG1 per ml Lysate，WB 1:3000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with His-tag fusion protein 3、IP (untransfected 293+anti-His mAb+ Protein G agarose) 4、IP (transfected 293+ normal Mouse IgG+Protein G agarose) 5、IP (transfected 293+anti-His mAb+ Protein G agarose) 6、Recombinant protein (E.coli) IF analysis of 293 cells transfected with a His-tag protein, using anti-HisTag Mouse mAb at a 1:1000 dilution (blue DAPI, red anti-His).