0.5ug HA fusion protein+ Primary antibody dilution at 1:10,000.

IP antibody use：5ug HA Mouse IgG1 per ml Lysate，WB 1:5000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with HA-tag fusion protein 3、IP(untransfted 293+anti-HA mAb+Protein G agarose) 4、IP (transfected 293+ normal Mouse IgG+Protein G agarose) 5、IP (transfected 293+anti-HA mAb+ Protein G agarose) 6、IP (transfected 293+Protein G) 7、Recombinant protein (E.coli). IF analysis of 293 cells transfected with a HA-tag protein, using anti-HATag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-HA)