1ug C-Myc fusion protein+ Primary antibody dilution at 1:5,000 (lane 1) and 1:10,000 (lane 2).

IP antibody use：3ug Flag Mouse IgG1 per ml Lysate，WB 1:5000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with C-Myc-tag fusion protein 3、IP (transfected 293+ normal Mouse IgG+Protein G agarose) 4、IP (transfected 293+anti- C-Myc mAb+ Protein G agarose) 5、IP (transfected 293+Protein G) 6、Recombinant protein (E.coli). IF analysis of 293 cells transfected with a C-Myc-tag protein, using anti-C-MycTag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-C-Myc).