IP antibody use:5ug Flag Mouse IgG1 per ml Lysate,WB 1:5000
1、untransfected 293 cell lysate
2、transfected 293 cell lysate with Flag-tag fusion protein
3、IP (transfected 293+ normal Mouse IgG+Protein G agarose)
4、IP (transfected 293+anti- Flag mAb+ Protein G agarose)
5、IP (transfected 293+Protein G)
6、Recombinant protein (E.coli).
IF analysis of 293 cells transfected with a Flag-tag protein, using anti-FlagTag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-Flag).