-20ºC for 1 year. Avoid repeated freeze/defrost cycles.
Purification:
Affinity purified
Reactivity:
HumanPredicted: rat, mus
Not yet tested in other species.
Source:
Rabbit polyclonal ATG4D (1) antibody was raised against a recombinate human ATG4D protein 241-421aa (BC068992).
Applications:
For research use only
ELISA titer: 1:20,000-1:80,000
Western blot analysis: 1:200-1:1,000
Immunohistochemistry: 1:100-1:500
Optimal dilutions/concentrations should be determined by the end user.
References:
(1) Betin,V.M., MacVicar,T.D., Parsons,S.F., et al.A cryptic mitochondrial targeting motif in Atg4D links caspase cleavage with mitochondrial import and oxidative stress. Autophagy 8 (4), 664-676 (2012)