-20ºC for 1 year. Avoid repeated freeze/defrost cycles.
Purification:
Affinity purified
Reactivity:
HumanPredicted: rat, mus
Not yet tested in other species.
Source:
Rabbit polyclonal ATG5(1) antibody was raised against a recombinate human ATG5 protein 94-275aa (BC002699).
Applications:
For research use only
ELISA titer: 1:20,000-1:80,000
Western blot analysis: 1:200-1:1,000
Immunohistochemistry: 1:100-1:500
Optimal dilutions/concentrations should be determined by the end user.
References:
(1) Otomo,C., Metlagel,Z., Takaesu,G. and Otomo,T. Structure of the human ATG12 ATG5 conjugate required for LC3 lipidation in autophagy. Nat. Struct. Mol. Biol. 20 (1), 59-66 (2013)