-20ºC for 1 year. Avoid repeated freeze/defrost cycles.
Purification:
Affinity purified
Reactivity:
HumanNot yet tested in other species.
Source:
Rabbit polyclonal APAF1 (1) antibody was raised against a peptide from N terminal residues of human APAF1 protein.
Applications:
For research use only
ELISA titer: 1:20,000-1:80,000
Western blot analysis: 1:1,000-1:2,000
Immunohistochemistry: 1:100-1:500
Optimal dilutions/concentrations should be determined by the end user.
References:
(1) Manns,J., Daubrawa,M., Driessen,S. Triggering of a novel intrinsic apoptosis pathway by the kinase inhibitor staurosporine: activation of caspase-9 in the absence of Apaf-1. FASEB J. 25 (9), 3250-3261 (2011)