-20ºC for 1 year. Avoid repeated freeze/defrost cycles.
Purification:
Affinity purified
Reactivity:
HumanPredicted: rat, mus
Not yet tested in other species.
Source:
Rabbit polyclonal LIN28 (1) antibody was raised against a recombinate human LIN28 protein 1-209aa (BC028566).
Applications:
For research use only
ELISA titer: 1:20,000-1:80,000
Western blot analysis: 1:1,000-1:2,000
Immunohistochemistry: 1:100-1:500
Optimal dilutions/concentrations should be determined by the end user.
References:
(1) Lightfoot,H.L., Bugaut,A., Armisen,J. et al. A LIN28-dependent structural change in pre-let-7g directly inhibits dicer processing. Biochemistry 50 (35), 7514-7521 (2011)