-20ºC for 1 year. Avoid repeated freeze/defrost cycles.
Purification:
Affinity purified
Reactivity:
HumanPredicted: mus, rat
Not yet tested in other species.
Source:
Rabbit polyclonal NEDD4 (1) antibody was raised against a recombinate human NEDD4 protein 634-878aa (NM_006154).
Applications:
For research use only
ELISA titer: 1:20,000-1:80,000
Western blot analysis: 1:1,000-1:2,000
Immunohistochemistry: 1:100-1:500
Optimal dilutions/concentrations should be determined by the end use
References:
(1) Simonin,A. and Fuster,D. Nedd4-1 and beta-arrestin-1 are key regulators of Na+/H+ exchanger 1 ubiquitylation, endocytosis, and function. J. Biol. Chem. 285 (49), 38293-38303 (2010)