-20ºC for 1 year. Avoid repeated freeze/defrost cycles.
Purification:
Affinity purified
Reactivity:
HumanPredicted: mus, rat
Not yet tested in other species.
Source:
Rabbit polyclonal E2F1 (1) antibody was raised against a recombinate human E2F1 protein 183-433aa (NM_005225).
Applications:
For research use only
ELISA titer: 1:20,000-1:80,000
Western blot analysis: 1:500-1:1,000
Immunohistochemistry: 1:50-1:200
Optimal dilutions/concentrations should be determined by the end user.
References:
(1) Paik,J.C., Wang,B., Liu,K., et al. Regulation of E2F1-induced apoptosis by the nucleolar protein RRP1B. J. Biol. Chem. 285 (9), 6348-6363 (2010)