-20ºC for 1 year. Avoid repeated freeze/defrost cycles.
Purification:
Affinity purified
Reactivity:
Human
Not yet tested in other species.
Source:
Rabbit polyclonal pdk1(1) antibody was raised against a 16 amino acid peptide from near the C terminal residues of human pdk1 protein.
Applications:
For research use only
Peptide ELISA titer: 1:20,000-1:80,000
Western blot analysis: 1:200-500
Optimal dilutions/concentrations should be determined by the end user.
References:
(1) Yang,K.J., Shin,S., Piao,L.,et al. Regulation of 3-phosphoinositide-dependent protein kinase-1 (PDK1) by Src involves tyrosine phosphorylation of PDK1 and Src homology 2 domain binding. J. Biol. Chem. 283 (3), 1480-1491 (2008).