-20ºC for 1 year. Avoid repeated freeze/defrost cycles.
Purification:
Affinity purified
Reactivity:
Human
Not yet tested in other species.
Source:
Rabbit polyclonal PARP(1) antibody was raised against a 15 amino acid peptide from the N terminal residues of human PARP protein.
Applications:
For research use only
Peptide ELISA titer: 1:20,000
Western blot analysis: 1:200-1,000
Immunohistochemistry: 1:50-200
Optimal dilutions/concentrations should be determined by the end user.
References:
(1) Simonin,F., Menissier-de Murcia,J., Poch,O et,al Expression and site-directed mutagenesis of the catalytic domain of human poly(ADP-ribose)polymerase in Escherichia coli. Lysine 893 is critical for activity. J. Biol. Chem.1990;265 (31):19249-19256.