-20ºC for 1 year. Avoid repeated freeze/defrost cycles.
Purification:
Affinity purified
Reactivity:
Human, mus, rat
Not yet tested in other species.
Source:
Rabbit polyclonal Bub3 (1) antibody was raised against a peptide from the N terminal residues of human Bub3 protein.
Applications:
For research use only
Peptide ELISA titer: 1:10,000-1:40,000
Immunohistochemistry: 1:50-200
Western blot analysis: 1:200-1,000
Optimal dilutions/concentrations should be determined by the end user.
References:
(1) Meierhofer,D., Wang,X., Huang,L. and Kaiser,P. Quantitative analysis of global ubiquitination in HeLa cells by mass spectrometry. J. Proteome Res. 7 (10), 4566-4576 (2008)