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Western blot analysis of extracts of various cell lines, using CDH6 antibody (E98109) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using CDH6 antibody (E98109) at dilution of 1:100 (40x lens).