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Western blot analysis of extracts of various cell lines, using ZBP1 antibody (E95001) at 1: 1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H +L) at 1: 10000 dilution. Lysates / proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.