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Western blot analysis of extracts of various cell lines, using RAC3 antibody (E94067) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
Immunofluorescence analysis of A549 cells using RAC3 antibody (E94067). Blue: DAPI for nuclear staining.