• Catalog Number:

  E92361

• Amount:

  100ul

• Form of Antibody:

  Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Storage/Stability:

  Store at -20oC or -80oC. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• class="eg_cn_title"Immunogen:

  A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3

• Purification:

  Affinity purification

• Reactivity:

  H M ROther (Wide Range)

• SwissProtNo:

  Q16695

• Source:

  Rabbit

• Applications:

  WBIHC IF

• Alter Name:

  HIST1H3J; H3/j ;H3FJ ; Histone H3.1; Histone H3/a; Histone H3/b;Histone H3/c; Histone H3/d; Histone H3/f;Histone H3/h ; Histone H3/I;HistoneH3/j ;Histone H3/k; Histone H3/l;HIST3H3;

• References:

  1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79. 2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41. 3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5. 4. Cheung, P. et al. (2000) Cell 103, 263-71. 5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73. 6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9. 7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13. 8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60. 9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9. 10. Preuss, U. et al. (2003) Nucleic Acids Res 31, 878-85. 11. Dai, J. et al. (2005) Genes Dev 19, 472-88. WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Western blot analysis of extracts of HeLa cellline and H3 protein expressed in E.coli., usingH3K27me1antibody.

Dot-blot analysis of all sorts of methylation peptides using H3K27me1 antibody. Immunofluorescence analysis of 293T cell using H3K27me1 antibody.