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Western blot analysis of extracts of various cell lines, using IPO5 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunofluorescence analysis of U2OS cells using IPO5 antibody .Blue: DAPI for nuclear staining.