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Western blot analysis of extracts of various cell lines, using SP140 antibody (E917634) at 1: 1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H + L) at 1: 10000 dilution. Lysates / proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.