• Catalog Number:

  E916208

• Amount:

  100ul

• Form of Antibody:

  Store at -20 ℃. Avoid freeze / thaw cycles.

• Storage/Stability:

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Positive sample: HeLa, Jurkat, DU145, HT-29, mouse testis

• class="eg_cn_title"Immunogen:

  Recombinant fusion protein containing a sequence corresponding to amino acids 781-910 of human RAD54B (NP_036547.1).Sequence: RQISKQGLCGAVVDLTKTSEHIQFSVEELKNLFTLHESSDCVTHDLLDCECTGEEVHTGD SLEKFIVSRDCQLGPHHQKSNSLKPLSMSQLKQWKHFSGDHLNLTDPFLERITENVSFIFQNITTQATGT

• Purification:

  method: Affinity purification

• SwissProtNo:

  Q9Y620

• Source:

  Rabbit

• Applications:

  WB 1: 500-1: 2000 ; IF 1:50-1: 200; IP 1:50-1: 100.Theoretical molecular weight: 103kDa Actual molecular weight: 103kDa

Western blot analysis of extracts of various cell lines, using RAD54B antibody(E916208) at 1: 1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H + L) (AS014) at 1: 10000 dilution. Lysates / proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.