Store at -20 ℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Positive sample:
A549, HepG2, Mouse kidney, LO2
Cell localization:
Secreted, extracellular matrix, extracellular space
Storage/Stability:
Store at -20°C . Avoid freeze / thaw cycles.
class="eg_cn_title"Immunogen:
Recombinant protein of human ECM1
Purification:
Affinity purified
SwissProtNo:
Q16610
Alias:
ECM1; URBWD; extracellular matrix protein 1
Reactive species:
Human, Mouse, Rat
Verify the application:
WB, IHC
Recommended dilution ratio:
WB 1: 500-1 : 1000;IHC 1: 50-1: 100
Source:
Rabbit
Western blot analysis of extracts of various cell lines, using ECM1 antibody (E91513).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H + L) at 1: 10000 dilution.
Lysates proteins: 25ug per lane. Blocking buffer : 3% nonfat dry milk in TBST.