PBS with 0.02% sodium azide, 50% glycerol, pH7.3.experimental method:Western blotting
Positive sample:Mouse brain, Rat brain
Cell localization:Cell membrane, Cytoplasm, Multi-pass membrane protein
class="eg_cn_title"Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human OPRM1 (NP_000905.3).Sequence:IYVIIKALVTIPETTFQTVSWHFCIALGYTNSCLNPVLYAFLDENFKRCFREFCIPTSSNIEQQNSTRIRQNTRDHPSTANTVDRTNHQLENLEAETAPLP
SwissProtNo:
P35372Alias:OPRM1; LMOR; M-OR-1; MOP; MOR; MOR1; OPRM; opioid receptor mu 1
Reactive species:Mouse, Rat
Verify the application:WB
Recommended dilution ratio:WB 1: 500-1: 1000
Theoretical molecular weight:10-20kDa / 33-55kDa
Actual molecular weight:72kDa
Source:
Rabbit
Western blot analysis of extracts of various cell lines using OPRM1 antibody (E90456). Secondary antibody: HRP Goat Anti-Rabbit IgG (H + L) at 1: 10000 dilution. Lysates / proteins: 25ug per lane. Blocking buffer : 3% nonfat dry milk in TBST.