• Catalog Number:

  E8ET1608-47

• Amount:

  100ul

• Form of Antibody:

  Liquid at 0.5 mg/ml in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Storage/Stability:

  Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• class="eg_cn_title"Immunogen:

  Recombinant protein

• Reactivity:

  Human,Mouse,Rat

• Applications:

  WB,ICC/IF,IHC-P

• Other Names:

  Calcium/calmodulin dependent protein kinase II alpha antibody; Calcium/calmodulin dependent protein kinase II beta antibody; Calcium/calmodulin dependent protein kinase II delta antibody; Calcium/calmodulin dependent protein kinase II gamma antibody; Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody; CaM kinase II alpha antibody; CaM kinase II antibody; CaM kinase II beta antibody; CaM kinase II delta antibody; CaM kinase II gamma antibody; CaM kinase II subunit alpha antibody; CaMK-II subunit alpha antibody; CAMK2 antibody; Camk2a antibody; CAMK2B antibody; CAMK2D antibody; CAMK2G antibody; CAMKA antibody; KCC2A_HUMAN antibody

Western blot analysis of CaMKⅡ on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY-5Y cell lysate; Lane 2: PC-12 cell lysate; Lane 3: SHG-44 cell lysate.

ICC staining of CaMKⅡ in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Immunohistochemical analysis of paraffin- embedded rat brain tissue using anti-CaMKⅡ antibody. Counter stained with hematoxylin.