Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
class="eg_cn_title"Immunogen:
affinity purified
Reactivity:
Human Mouse
SwissProtNo:
O75473(human) Q9Z1P4(mouse)
Source:
Rabbit
Applications:
WB: 1:1,000-1:2,000 ICC: 1:50
Specificity Sensitivity:
This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region of LGR5.
References:
1. Identification of overexpression of orphan G protein-coupled receptor GPR49 in human colon and ovarian primary tumors."McClanahan T., Koseoglu S., Smith K., Grein J., Gustafson E., Black S., Kirschmeier P., Samatar A.A.Cancer Biol. Ther. 5:419-426(2006)
2. "Immunostaining of Lgr5, an intestinal stem cell marker, in normal and premalignant human gastrointestinal tissue."Becker L., Huang Q., Mashimo H.ScientificWorldJournal 8:1168-1176(2008)
Western blot analysis on F9 (A)
and NCCIT (B) cell lysates using anti-LGR5 rabbit polyclonal antibody.
ICC image of LGR5 antibody stained NCCIT cells. The secondary antibody (green) was goat anti-rabbit IgG (H+L) FITC conjugated.