Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
class="eg_cn_title"Immunogen:
affinity purified
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P68431(human)
Source:
Rabbit
Applications:
WB: 1:1,000~1:2000
Specificity Sensitivity:
This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region of Histone H3.
References:
1. Flanagan J.F., Mi L.-Z., Chruszcz M., Cymborowski M., Clines K.L., Kim Y., Minor W., Rastinejad F., Khorasanizadeh S."Double chromodomains cooperate to recognize the methylated histone H3 tail."Nature 438:1181-1185(2005)
2. "Arginine methylation of the histone H3 tail impedes effector binding."Iberg A.N., Espejo A., Cheng D., Kim D., Michaud-Levesque J., Richard S., Bedford M.T.J. Biol. Chem. 283:3006-3010(2008)
Western blot analysis on
A:HepG2
B:NIH/3T3
C:Rat liver
cell lysates using anti-Histone H3 polyclonal antibody.