Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
class="eg_cn_title"Immunogen:
affinity purified
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P60709 (human) P60710 (mouse)
Source:
Rabbit
Applications:
WB: 1:1,000~1:2,000
Specificity Sensitivity:
This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region ofβ-actin.
References:
1. Ponte P., Ng S.Y., Engel J., Gunning P., Kedes L."Evolutionary conservation in the untranslated regions of actin mRNAs: DNA sequence of a human beta-actin cDNA." Nucleic Acids Res. 12:1687-1696(1984)
2. Ohmori H., Toyama S., Toyama S."Direct proof that the primary site of action of cytochalasin on cell motility processes is actin."J. Cell Biol. 116:933-941(1992)
Western blot analysis on
A:Hela
B:NIH/3T3
C:PC12
cell lysates using anti-β-actin polyclonal antibody.