Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
class="eg_cn_title"Immunogen:
affinity purified
Reactivity:
Human
SwissProtNo:
P01106
Source:
Rabbit
Applications:
WB: 1:500
Specificity Sensitivity:
This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the residues of c-myc.
References:
1. "A quantitative atlas of mitotic phosphorylation."Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008)
2. "Transactivation of gene expression by Myc is inhibited by mutation at the phosphorylation sites Thr-58 and Ser-62."Gupta S., Seth A., Davis R.J. Proc. Natl. Acad. Sci. U.S.A. 90:3216-3220(1993)
Western blot analysis on myc-tagged recombinant protein with (A) and A549 cell lysates (B).
ICC/IF image of c-myc antibody stained human A549 cells. The secondary antibody (green) was goat anti-rabbit IgG (H+L) FITC conjugated.