-
Western blot analysis of extracts of various cell lines using NR0B2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat liver using NR0B2 antibody at dilution of 1:100 .
Immunofluorescence analysis of U2OS cells using NR0B2 antibody . Blue: DAPI for nuclear staining.