Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage/Stability:
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 μg/ml BSA and 50% glycerol. Stable for at least 6-months if stored at –20°C.
Purification:
Affinity purified
Source:
Purification: Polyclonal antibodies are produced by immunizing rabbits with a bacterially expressed human Rheb. Antibodies are purified by protein A chromatography.
Applications:
Species Cross-Reactivity* Molecular Wt. SourceWB, IP H, Mu, R, Monkey ~21 Kd Rabbit Polyclonal
* Based on sequence homology
Recommended Antibody Dilutions:
Immuoprecipitation: 1:100-200;
Western Blotting: 1:1,000-2,000. Antibody diluent: PBST-5% non-fat milk or BSA.
Specificity Sensitivity:
Rheb Antibody detects endogenous levels of total Rheb on Western analysis and do not cross-react with other Ras family proteins.
