Mouse Monoclonal (IgG1)
1mg/ml in PBS, pH 7.4 with 0.05% sodium azide. Frozen liquid.
Storage/Stability:
Stable for 1 year at -20C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4C for 3 months.
class="eg_cn_title"Immunogen:
Beta-cytoplasmic actin N-terminal peptide.
Purification:
Affinity purified
Reactivity:
Human, bovine, sheep, pig, rabbit, feline, dog, mouse, rat, guinea pig, chicken, and carp.
Applications:
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows: Western blot at dilution of 1:1000-4000. It is very useful as a loading control for quantitative western blotting. The antibody recognizes a 43kDa band in a Western Blot analysis. Immunofluorescence staining at 1:100 dilutions.
Specificity Sensitivity:
Recognize 43KDa beta-actin from Human
References:
Lee CS, et al. (2004) Exp Mol Med,36 (6):582-587.
McElhinny AS, et al. (2005) J Cell Biol, 170 (6): 947-957.