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WB analysis of mouse-heart lysis using THBS2 antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000.
Immunohistochemical analysis of paraffin-embedded Human colon. Antibody was diluted at 1:200(4℃,overnight). High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. Secondary antibody was diluted at 1:200(room temperature, 30min).