• Catalog Number:

  E20-71241

• Amount:

  100ul

• Form of Antibody:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. .

• Storage/Stability:

  Stability: -20°C/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized peptide derived from human Cytochrome P450 7B1. AA range101-150

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human

• Source:

  Rabbit

• Applications:

  WB: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

• Other Names:

  CYP7B1; 25-hydroxycholesterol 7-alpha-hydroxylase; Cytochrome P450 7B1; Oxysterol 7-alpha-hydroxylase

• Specificity Sensitivity:

  CYP7B1 Polyclonal Antibody detects endogenous levels of CYP7B1 protein.

Immunohistochemical analysis of paraffin- embedded Human skeletal muscle. Antibody was diluted at 1:100(4°,overnight). High- pressure and temperature Tris- EDTA, pH8.0 was used for antigen retrieval. Negative control (right) obtained from antibody was pre-absorbed by immunogen peptide.

WB analysis of lysates from LOVO cells, using Cytochrome P450 7B1 Antibody. The lane on the right is blocked with the synthesized peptide.