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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°,overnight). High-pressure and temperature Tris- EDTA, pH8.0 was used for antigen retrieval. Negative control (right) obtained from antibody was pre- absorbed by immunogen peptide.
WB analysis of lysates from NIH/3T3 cells, using APC6 Antibody. The lane on the right is blocked with the synthesized peptide.
Expression: Aorta endothelial cell,Brain,Epithelium,Lung,Skin.