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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using BCL-2 Antibody. The picture on the right is blocked with the synthesized peptide.
WB analysis of various cells using primary antibody diluted at 1:1000 (4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour). Cell lysate was extracted by Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit.