• Catalog Number:

  E20-60564

• Amount:

  100ul

• Form of Antibody:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. .

• Storage/Stability:

  Stability: -20°C/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized peptide derived from human NEK9 around the phosphorylation site of Thr210. AA range176-225

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen..

• Reactivity:

  Human,Mouse,Rat

• Source:

  Rabbit

• Applications:

  WB,IHC-p,IF,ELISA

• Other Names:

  NEK9; KIAA1995; NEK8; NERCC; Serine/threonine-protein kinase Nek9; Nercc1 kinase; Never in mitosis A-related kinase 9; NimA-related protein kinase 9; NimA-related kinase 8; Nek8

• Specificity Sensitivity:

  Phospho-Nek9 (T210) Polyclonal Antibody detects endogenous levels of Nek9 protein only when phosphorylated at T210..

Immunohistochemical analysis of paraffin- embedded Human brain. Antibody was diluted at 1: 100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negative control (right) obtained from antibody was pre-absorbed by immunogen peptide.

Enzyme-Linked Immunosorbent Assay (Phospho- ELISA) for Immunogen Phosphopeptide (Phospho- left) and Non-Phosphopeptide (Phospho-right), using NEK9 (Phospho-Thr210) Antibody.

WB analysis of lysates from HepG2 cells, using NEK9 (Phospho-Thr210) Antibody. The lane on the right is blocked with the phospho peptide.