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WB analysis of lysates from 1) Hela, 2) 3T3, 3) PC12 cells, (Green) primary antibody was diluted at 1:1000, 4°over night, secondary antibody was diluted at 1:10000, 37° 1hour. (Red) Actin β Monoclonal Antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody was diluted at 1:10000, 37° 1 hour.
Immunohistochemical analysis of paraffin- embedded Mouse Heart Tissue using PPAR-γ Polyclonal Antibody.
WB analysis of 1) Hela, 2) 3T3, 3) PC12 using PPAR-γ Polyclonal Antibody. Secondary antibody was diluted at 1:20000.