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WB analysis of 1) Jurkat, 2) 293T, 3) C2C12, 4) Rat Kidney using Beclin-1 Polyclonal Antibody. Secondary antibody was diluted at 1:20000
WB analysis of lysates from 1) Jurkat, 2) 293T, 3) C2C12, 4) Rat Kidney cells, (Green) primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:RS23920)was diluted at 1:10000, 37° 1hour. (Red) Actin β Monoclonal Antibody(5B7) was diluted at 1:5000 as loading control, 4° over night,secondary antibody was diluted at 1:10000, 37° 1hour.