• Catalog Number:

  E1RA1-050

• Amount:

  500μg/500μl

• Form of Antibody:

  Liquid. The product is 1 mg/ml in 10 mM Phosphate buffered saline, pH 7.4 containing, 10% glycerol and 0.1% sodium azide as preservative.

• Storage/Stability:

  Store at -20°C. -80°C for long-term storage. Avoid exposure to light. Avoid repeated freezing and thawing.

• Source:

  Recombinant expressed in E. coli

• Applications:

  The protein is suitable as control reagent for RFP expression studies or as labeling reagent. Applications include: Flow cytometers, fluorescence microscopy, and microinjection, antibody purification, etc. at 1:100 dilution. Maximum absorption wavelength at 557 nm and emission wavelength at 585 nm.

• Specificity Sensitivity:

  Under optimal conditions, 1 mg RFP-rProtein A will bind approximately 5 mg IgG. Optimal binding of RFP-rProtein A to antibodies occurs at pH 5.0 to 8.0 and can be eluted over a pH range of 2.5 to 3.0.

Immunofluorescence staining of methanol-fixed HeLa cells using Synaptotagmin (Ab-309) Antibody (E021292) (Left) and PLC-γ1 (phospho-Tyr771) Antibody (E011523) (Right) consequently detected with RFP-rProtein A (Red).