• Catalog Number:

  E27D2019-P1

• Amount:

  50ug

• Source:

  2019nCoVExpression Host: Baculovirus-Insect Cells

• References:

  1.Shen S, et al. (2007) Expression, glycosylation, and modification of thespike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35. 2.Du L, et al. (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36. 3.Xiao X, et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30.

Predicted N terminal: Val 16 Molecular Mass:The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD, His tag) consists of 1209 amino acids and predicts a molecular mass of 134.36 kDa.

SDS-PAGE: Protein Description The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the of the infection process. It's been reported that 2019-nCoV can infect thehuman respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.