• Catalog Number:

  E42020T6

• Amount:

  100μg

• Form of Antibody:

  20mM Tris, 300mM NaCl, PH8.5

• Storage/Stability:

  For long term storage, store at -20~-70 °C, avoiding freeze/thaw cycles. 2019-nCoV N protein and its fragments are not stable and prone to aggregate at RT and 4 °C.

• Purification:

  Ni column

• Source:

  Isotope:2019-nCov

• Specificity Sensitivity:

  No non-specific binding to normal human serum was identified so far.

ELISA plate was coated by N protein, 100 µL/cell, at various concentrations. The direct ELISA analysis was performed by loading 100 µL per well of a anti C-ter His tag HRP conjugated mAb at a concentration of 1:2000. The plate was incubated for 1 hours at 37℃, then washed 5 time. Detection was performed using TMB substrate for 10 minutes at room temperature in the dark. The plate was stopped with 2M sulfuric acid. Absorbances were read on a spectrophotometer at 450 nm.