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Catalog Number:
E12-152 Amount:
100ul Form of Antibody:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Storage/Stability:
Store at -20℃/1 year Purification:
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. Reactivity:
N/A Applications:
WB: 1:5,000 IP: 1:200 Specificity Sensitivity:
The GFP tag antibody can recognize GFP,EGFP and GFP, EGFP tag fusion proteins. It may also recognize RFP, YFP, CFP.
Western blot analysis of GFP transfected Hela with E12-152 diluted at
1) 1:5,000
2) 1:10,000.
Western blot analysis of GFP transfected Hela with E12-152 diluted at 1:200
1) Input (control)
2) IP products, antibody dilution 1:200, Secondary antibody: Goat anti-Mouse IgG, Heavy chain specific(S002F), 1:5000.