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Catalog Number:
E12-138 Amount:
100ul Form of Antibody:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Storage/Stability:
Store at -20℃/1 year. Do not aliquot the antibody. Purification:
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. Reactivity:
H, R, M Specificity Sensitivity:
NFκB p65 Mouse monoclonal antibody detects endogenous p65 proteins.
Western blot analysis of 1) Hela, 2) Rat Heart Tissue, 3) Mouse Spleen Tissue with E12-138 diluted at 1:2000.
1、Input: Hela Cell Lysate
2、IP product: IP dilute 1:200
Western blot analysis: primary antibody : E12-138 1:2,000
Secondary antibody: Goat anti-Mouse IgG, Light chain specific(S003), 1:5,000
IF analysis of Hela with E12-138(Left) and DAPI (Right) diluted at 1:100.