Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year. Do not aliquot the antibody.
class="eg_cn_title"Immunogen:
KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis. The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. It is strictly associated with cell proliferation. During interphase, the Ki-67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes. Ki-67 protein is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0).
Purification:
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Reactivity:
H,M,R
Applications:
IHC: 1:200
Specificity Sensitivity:
The Ki 67 Mouse Monoclonal antibody detects endogenous Ki 67 proteins
IHC staining of Mouse Kidney tissue with Ki 67 mouse mAb(4A8) diluted at 1:200.