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Catalog Number:
E12-055 Amount:
100ul Form of Antibody:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Storage/Stability:
Store at -20℃/1 year. Do not aliquot the antibody. Purification:
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. Reactivity:
H, R, M Applications:
WB: 1:2,000-5,000 IF:1:100-500 IP:1:200 Specificity Sensitivity:
The Histone H3 antibody can detects endogenous Histone H3 protein.
. Western blot analysis of 1) Hela, 2) Raw, 3) Mouse Brain Tissue, 4) Rat Brain Tissue with E12-055 diluted at 1:5,000.
IF analysis of Hela with DAPI (Left) and E12-055(Right) diluted at 1:200.
1、Input: Hela Cell Lysate
2、IP product: IP dilute 1:200
Western blot analysis: primary antibody : E12-055 1:2,000
Secondary antibody: Goat anti-Mouse IgG(H+L) (S001) 1:10,000