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Catalog Number:
E12-048 Amount:
100ul Form of Antibody:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Storage/Stability:
Store at -20℃/1 year. Do not aliquot the antibody. Purification:
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. Reactivity:
H, R, M Applications:
WB: 1:5,000-10,000 IP:1:200 IF: 1:100-200 Specificity Sensitivity:
The α-tubulin antibody can detects endogenousα-tubulin protein
Western blot analysis of 1) Hela, 2) Rat BrianTissue, 3) Mouse Brain Tissue with E12-048 diluted at 1:5,000.
1、Input: Mouse Brain Tissue Lysate
2、IP product: IP dilute 1:200
Western blot analysis: primary antibody : E12-048 1:5,000 Secondary antibody: Goat anti-Mouse IgG, Light chain specific (S003), 1:5,000