Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human HDAC1 around the phosphorylation site of serine 421/423.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q13547
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity Sensitivity:
HDAC1 (Ab-421/423) antibody detects endogenous levels of total HDAC1 protein.
References:
Dephoure N, et al. (2008) Proc Natl Acad Sci U S A 105, 10762-7.
Han G, et al. (2008) Proteomics 8, 1346-61.
Molina H, et al. (2007) Proc Natl Acad Sci U S A 104, 2199-204.
Olsen JV, et al. (2006) Cell 127, 635-48.
Pflum MK, Tong JK, et al. (2001) J Biol Chem 276, 47733-41.
Marmorstein, R. et al. (2001) Cell. Mol. Life Sci. 58, 693-703.
Immunohistochemical analysis of paraffin- embedded human lung adenocarcinoma tissue using HDAC1 (Ab-421/423) Antibody (E022085).